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pgem-4  (Promega)

 
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    Promega pgem-4
    Pgem 4, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgem+4/pmc10876878-67-31-5?v=Promega
    Average 90 stars, based on 1 article reviews
    pgem-4 - by Bioz Stars, 2026-07
    90/100 stars

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    Bacterial strains and plasmids used in the study

    Journal: Applied and Environmental Microbiology

    Article Title: Alcoholytic Cleavage of Polyhydroxyalkanoate Chains by Class IV Synthases Induced by Endogenous and Exogenous Ethanol

    doi: 10.1128/AEM.03576-13

    Figure Lengend Snippet: Bacterial strains and plasmids used in the study

    Article Snippet: To remove the phaAB Re genes from pGEM- phaRC YB-4 AB , the plasmid was digested with SalI and NruI and self-ligated using a BKL kit (TaKaRa Bio Inc., Otsu, Japan) to yield pGEM- phaRC YB-4 .

    Techniques: Plasmid Preparation

    Effect of ethanol on P(3HB) molecular weight produced by E. coli JM109 expressing PhaRC YB-4 (pBBR1- phaRC YB-4 AB ) and PhaRC Bm (pGEM- phaRC Bm AB ) in two-stage culture a

    Journal: Applied and Environmental Microbiology

    Article Title: Alcoholytic Cleavage of Polyhydroxyalkanoate Chains by Class IV Synthases Induced by Endogenous and Exogenous Ethanol

    doi: 10.1128/AEM.03576-13

    Figure Lengend Snippet: Effect of ethanol on P(3HB) molecular weight produced by E. coli JM109 expressing PhaRC YB-4 (pBBR1- phaRC YB-4 AB ) and PhaRC Bm (pGEM- phaRC Bm AB ) in two-stage culture a

    Article Snippet: To remove the phaAB Re genes from pGEM- phaRC YB-4 AB , the plasmid was digested with SalI and NruI and self-ligated using a BKL kit (TaKaRa Bio Inc., Otsu, Japan) to yield pGEM- phaRC YB-4 .

    Techniques: Molecular Weight, Produced, Expressing

    In vitro incubation of the P(3HB)–PhaRC YB-4 complex in phosphate buffer with or without ethanol a

    Journal: Applied and Environmental Microbiology

    Article Title: Alcoholytic Cleavage of Polyhydroxyalkanoate Chains by Class IV Synthases Induced by Endogenous and Exogenous Ethanol

    doi: 10.1128/AEM.03576-13

    Figure Lengend Snippet: In vitro incubation of the P(3HB)–PhaRC YB-4 complex in phosphate buffer with or without ethanol a

    Article Snippet: To remove the phaAB Re genes from pGEM- phaRC YB-4 AB , the plasmid was digested with SalI and NruI and self-ligated using a BKL kit (TaKaRa Bio Inc., Otsu, Japan) to yield pGEM- phaRC YB-4 .

    Techniques: In Vitro, Incubation